AIE-GA is a fluorescent probe for the Golgi apparatus (GA) that operates in the green channel with an excitation wavelength of 405 nm and an emission range of 500-700 nm. It exhibits a strong binding affinity to interact with COX-2 by binding to its cyclooxygenase catalytic site [1].

For Golgi apparatus staining, HeLa cells are initially incubated with ACQ-GA (2 μM) and AIE-GA (2 μM). Golgi Tracker Red (333 μg/mL) is then added, and incubation is carried out at 37°C for 30 minutes. The medium is removed, and cells are washed three times with phosphate-buffered saline (PBS) before imaging under a confocal microscope [1]. The fluorescence of HeLa cells stained with ACQ-GA (2 μM), AIE-GA (2 μM), and Golgi Tracker Red (333 μg/mL) decreases with increased laser exposure [1].