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Anti-B2M Monoclonal Antibody-AF488
Catalog No. TMAZ-0010A4
Alias Beta-2-microglobulin
Anti-B2M Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting B2M. Anti-B2M Monoclonal Antibody-AF488 can be used in ELISA, FACS.
Anti-B2M Monoclonal Antibody-AF488
Anti-B2M Monoclonal Antibody-AF488
Catalog No. TMAZ-0010A4Alias Beta-2-microglobulin
Anti-B2M Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting B2M. Anti-B2M Monoclonal Antibody-AF488 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $345 | 12 days | |
| 50 μg | $575 | 12 days | |
| 100 μg | $865 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-B2M Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting B2M. Anti-B2M Monoclonal Antibody-AF488 can be used in ELISA, FACS. |
| Alias | Beta-2-microglobulin |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of B2M overexpressed 406F cells with TMAZ-0010A4, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of B2M protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed: 25356553) |
| Conjucates | AF488 |
| Others Formats | Biotin/APC/PE/AF555/AF647 |
| Antibody Types Available | 2 |
| Immunogen | B2M |
| Antigen Species | Human |
| Gene ID | 567 |
| Uniprot ID | |
| Biology Area | Immunology Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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