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Anti-IL7 Monoclonal Antibody-AF555
Catalog No. TMAZ-0206A5
Alias Interleukin-7
Anti-IL7 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting IL7. Anti-IL7 Monoclonal Antibody-AF555 can be used in ELISA, FACS.
Anti-IL7 Monoclonal Antibody-AF555
Anti-IL7 Monoclonal Antibody-AF555
Catalog No. TMAZ-0206A5Alias Interleukin-7
Anti-IL7 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting IL7. Anti-IL7 Monoclonal Antibody-AF555 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $570 | 12 days | |
| 50 μg | $945 | 12 days | |
| 100 μg | $1,415 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-IL7 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting IL7. Anti-IL7 Monoclonal Antibody-AF555 can be used in ELISA, FACS. |
| Alias | Interleukin-7 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of IL7 overexpressed 490F cells with TMAZ-0206A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of IL7 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed: 25870237, PubMed: 7527823). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG (PubMed: 8128231). Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5 (PubMed: 18523275, PubMed: 20974963) |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 1 |
| Immunogen | IL7 |
| Antigen Species | Human |
| Gene ID | 3574 |
| Uniprot ID | |
| Biology Area | Drug Target |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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