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Anti-PSAP Monoclonal Antibody-AF488
Catalog No. TMAZ-0240A4
Alias Prosaposin, Proactivator polypeptide
Anti-PSAP Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting PSAP. Anti-PSAP Monoclonal Antibody-AF488 can be used in ELISA, FACS, IF.
Anti-PSAP Monoclonal Antibody-AF488
Anti-PSAP Monoclonal Antibody-AF488
Catalog No. TMAZ-0240A4Alias Prosaposin, Proactivator polypeptide
Anti-PSAP Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting PSAP. Anti-PSAP Monoclonal Antibody-AF488 can be used in ELISA, FACS, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $570 | 12 days | |
| 50 μg | $945 | 12 days | |
| 100 μg | $1,415 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PSAP Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting PSAP. Anti-PSAP Monoclonal Antibody-AF488 can be used in ELISA, FACS, IF. |
| Alias | Prosaposin, Proactivator polypeptide |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of PSAP overexpressed 335F cells with TMAZ-0240A4, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS, IF |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of PSAP protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate|Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases|Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12)|Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling|Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases |
| Conjucates | AF488 |
| Others Formats | Biotin/APC/PE/AF555/AF647 |
| Antibody Types Available | 3 |
| Immunogen | PSAP |
| Antigen Species | Human |
| Gene ID | 5660 |
| Uniprot ID | |
| Biology Area | Other Functions |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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