Azide cyanine dye 728 serves as a multifunctional dye, essential in biological experiments for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its applications span from fundamental scientific research to a broad spectrum of clinical diagnostics. Additionally, dyes are utilized in traditional areas like textile dyeing and emerging fields such as functional textile processing, food pigments, and dye-sensitized solar cells.