BODIPY493/503 methyl bromide, a BODIPY dye, is a small molecule with strong ultraviolet absorption, a sharp fluorescence peak, and high quantum yield. It exhibits relative insensitivity to environmental polarity and pH while maintaining stability under various physiological conditions. The structural asymmetry of BODIPY allows for diverse structural derivatives. BODIPY lipid droplet dyes effectively permeate the cell membrane, localize polar lipids, and specifically stain lipid droplets, making them suitable for labeling both live and fixed cells [1]. Maximum excitation/emission wavelength: 493/503 nm [1].

BODIPY493/503 methyl bromide: Usage Instructions

1. Preparation of BODIPY493/503 Methyl Bromide Stock Solution
1.1 Prepare a stock solution by dissolving 1 mg of BODIPY493/503 methyl bromide with 293.3 μL anhydrous DMSO to achieve a concentration of 10 mM. Note: It is recommended to aliquot the stock solution and store it protected from light at -20°C or -80°C.

1.2 Preparation of Working Solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a BODIPY493/503 methyl bromide working solution at a concentration of 1-10 μM. Note: Adjust the working solution concentration as needed and prepare fresh immediately before use.

2. Cell Staining (Suspension Cells)
2.1 Centrifuge to collect the cells, wash twice with PBS for 5 minutes each time. Maintain cell density at 1×10^6/mL.
2.2 Add 1 mL of BODIPY493/503 methyl bromide working solution and incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400g for 3-4 minutes and discard the supernatant.
2.4 Wash cells twice with PBS for 5 minutes each time.
2.5 Resuspend cells with 1 mL of serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.

3. Cell Staining (Adherent Cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove coverslips from culture medium and aspirate excess medium.
3.3 Add 100 μL of dye working solution, gently agitate to cover cells completely, and incubate for 5-30 minutes.
3.4 Aspirate the dye working solution and wash cells 2-3 times with medium for 5 minutes each before observation using a fluorescence microscope or flow cytometer.

Storage Conditions
Store at -20℃, protected from light for up to one year.

Precautions
1. Adjust BODIPY493/503 methyl bromide working solution concentration and incubation time as necessary.
2. A positive control is recommended; incubate control cells with 30 μM oleic acid for 8 hours before proceeding with experiments.
3. This product is intended for scientific research by professionals only and is not suitable for clinical diagnosis, treatment, food, or drug use.
4. For your safety and health, wear lab coats and disposable gloves during handling.