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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | Inquiry | Inquiry | |
50 mg | Inquiry | Inquiry |
Description | BODIPY TR methyl ester serves as a lipophilic GFP counterstain, easily permeating cell membranes to accumulate in endomembranous organelles, while exhibiting minimal localization in plasma membranes. It is a highly effective red fluorescent vital dye (Ex=568 nm, Em=625 nm) that can reveal the location and shape of cell nuclei, the morphology of cells in embryonic tissues, and the boundaries of organ-forming tissues within an entire embryo [1]. |
In vitro | BODIPY TR methyl ester dye: Solubilized into anhydrous DMSO as a 5 mM stock solution. The stock DMSO solution is diluted 1:50 into Embryo Rearing Medium (ERM) buffered with 5 mM HEPES (pH 7.2), creating a final labeling solution of 100 μM BODIPY TR methyl ester dye with 2% DMSO, stored at -20°C. Embryos are stained in 100 μM BODIPY TR methyl ester dye for 1 h, followed by three successive washes in HEPES-buffered ERM. Embryos are then mounted in an open-faced chamber for imaging. For time-lapse recordings, vitally stained embryos are deyolked and secured to a coverslip. Embryos used for fixation experiments are fixed in 4% paraformaldehyde in HEPES-buffered ERM at 4°C for 1 h, then washed in ERM and mounted for confocal imaging. Samples are analyzed on a confocal microscope (with a 640 × 480 pixel frame buffer and an air-cooled Ar-Kr laser), co-exciting GFP and BODIPY TR methyl ester dye using two wavelengths of the major Ar-Kr laser lines (488 and 568 nm) simultaneously. |
Molecular Weight | 438.25 |
Formula | C22H17BF2N2O3S |
Cas No. | 150152-63-9 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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