C.I. Acid Black 94 is a multifunctional dye used in both traditional fields like textile dyeing and emerging fields such as functional textile processing, food pigments, and dye-sensitized solar cells. In biological experiments, dyes are critical tools that help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Their applications range from basic scientific research to clinical diagnostics.