Shopping Cart

Remove All
Your shopping cart is currently empty

DAF-FM DA

Name: DAF-FM DA
Brand: TargetMol
SKU: T18965
Price: 30 USD
Availability: InStock
Rating: 4.5 (1 reviews)

Catalog No. T18965Cas No. 254109-22-3

Alias Diaminofluorescein-FM diacetate

DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).

DAF-FM DA

Catalog No. T18965Alias Diaminofluorescein-FM diacetateCas No. 254109-22-3

DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).

Pack Size	Price	Availability	Quantity
1 mg	$898	35 days

Bulk & Custom

Add to Cart

Questions

Select Batch

Resource Download

COA

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).
In vitro	BCa cells were pre-incubated with 5 μM DAF-FM DA for 30 minutes to load the probe. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells. [1]
In vivo	Using zebrafish embryo sections live-stained with DAF-FM DA (5 μM), it was confirmed that the fluorescent signal was primarily localized to areas of ongoing bone formation.[2]
Alias	Diaminofluorescein-FM diacetate

Chemical Properties

Molecular Weight	496.42
Formula	C₂₅H₁₈F₂N₂O₇
Cas No.	254109-22-3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

DAF FM DADiaminofluorescein-FMDAF-FM DADAFFM DA