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DAF-FM DA
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Catalog No. T18965Cas No. 254109-22-3
Alias Diaminofluorescein-FM diacetate
DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).
DAF-FM DA
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Catalog No. T18965Alias Diaminofluorescein-FM diacetateCas No. 254109-22-3
DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 1 mg | $898 | 35 days |
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Product Introduction
Bioactivity
Chemical Properties
| Description | DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm). |
| In vitro | BCa cells were pre-incubated with 5 μM DAF-FM DA for 30 minutes to load the probe. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells. [1] |
| In vivo | Using zebrafish embryo sections live-stained with DAF-FM DA (5 μM), it was confirmed that the fluorescent signal was primarily localized to areas of ongoing bone formation.[2] |
| Alias | Diaminofluorescein-FM diacetate |
| Molecular Weight | 496.42 |
| Formula | C25H18F2N2O7 |
| Cas No. | 254109-22-3 |
| Smiles | O=C1OC2(C3=CC(F)=C(OC(=O)C)C=C3OC4=CC(OC(=O)C)=C(F)C=C42)C5=CC=C(NC)C(N)=C15 |
| Relative Density. | 1.56 g/cm3 (Predicted) |
Storage & Solubility Information
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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