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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | Inquiry | Inquiry | |
50 mg | Inquiry | Inquiry |
Description | DAz-2 is a cell-permeable sulfonic acid probe that can directly detect sulfonic acid-modified proteins in living cells [1]. |
In vitro | DAz-2 is utilized for detecting sulfonic acids under various conditions [1]: 1. Prepare 10 μM protein, 50 μg cellular lysate, or 200 μg protein lysate. 2. Add 50 or 100 μM H2O2 and incubate at room temperature for 20 minutes; then add 1 mM Daz-2 or 5% (v/v) DMSO, and react at room temperature for 15 minutes. 3. Quench the reaction with 5 mM DTT and remove excess reagents via gel filtration. 4. Separate proteins using 4-12% SDS-PAGE, transfer to a PVDF membrane, and detect using HRP-Streptavidin Western Blotting. |
Alias | DCP-N3 |
Molecular Weight | 195.22 |
Formula | C9H13N3O2 |
Cas No. | 1176905-54-6 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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