FAK-IN-20 (Compound 7b), a potent FAK inhibitor, demonstrates an IC 50 of 0.27 nM and exhibits anticancer properties. This compound arrests the cell cycle in the G2/M phase and induces cell apoptosis (apoptosis) through the generation of ROS [1].

FAK-IN-20 (Compound 7b) exhibits anticancer activity by inhibiting cell proliferation in human tumor cell lines such as HCT116 (colon cancer), A549 (lung cancer), MDA-MB-231 (breast cancer), and HeLa (cervical cancer), with IC50 values of 0.58, 0.75, 1.18, and 1.4 μM, respectively [1]. In HCT116 cells, FAK-IN-20 (0.2-2 μM; 24 h) causes cell cycle arrest at the G2/M phase and induces apoptosis through ROS generation (1-125 μM; 12 h) and apoptosis induction (0.2-25 μM; 12 h) [1]. Additionally, FAK-IN-20 (1 μM; 24 h) can inhibit cell migration in HCT116 cells [1].