IACS-9571 (trifluoroacetate) serves as an inhibitor of the bromodomain proteins TRIM24 and BRPF1, exhibiting an IC50 of 8 nM and a Kd of 31 nM against TRIM24, and a Kd of 14 nM against BRPF1.

IACS-9571 (trifluoroacetate) exhibits potent cellular activity with an EC50 value of 50 nM and demonstrates significant inhibitory effects on a spectrum of 32 bromodomains at a concentration of 1 μM. As a selective dual inhibitor of TRIM24/BRPF1, IACS-9571 (trifluoroacetate) achieves dissociation constants (Kd) of 1.3 nM and 2.1 nM, respectively, offering 9-fold and 21-fold selectivity over BRPF2 and BRPF3. Additionally, this compound does not affect the BET sub-family of bromodomains, showing exceptional selectivity greater than 7,700-fold when compared to BRD4 (1, 2) in relation to TRIM24.