IR 813 tosylate is a near-infrared (NIR) fluorescent dye with excitation and emission wavelengths of 815 nm and 840 nm, respectively, used for visualizing regional lymph nodes in mice [1].

IR 813 tosylate, at a concentration of 17.3 μM applied for 2 hours, induces 31.4% hemolysis in red blood cells [1]. The same compound, when used at a 5.9 μM concentration over a period of 24 hours, causes 50% cell death in MRC-5 cells [1].

IR 813 tosylate achieves peak fluorescence intensity at four hours post-injection and exhibits quick extravasation, facilitating the visualization of regional lymph nodes in mice [1]. Recommended protocol guidelines suggest personalizing the procedure to meet individual experimental requirements [1].