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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | Inquiry | Inquiry | |
50 mg | Inquiry | Inquiry |
Description | Mag-indo-1/AM, a cell-permeable fluorescent indicator for Mg2+ and Ca2+ (Ex=340-390 nm, Em=410-490 nm), is used to determine intracellular Mg2+ and Ca2+ concentrations [1][2]. |
In vitro | Measurement of intracellular Ca 2+: Incubate 3 mL cell suspension with 10 μM Mag-indo-1/AM for 15 min at 30℃. Centrifuge cells for 1 min at 500 rpm, resuspend in 5 mL isolation buffer (containing 1 mM CaCl 2), add 1 mM probenecid to prevent dye leakage, and store (Mag-indo-1/AM-loaded cells) at room temperature. Incubate at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge, resuspend, and store at room temperature. Place a small portion of loaded cells in an experimental chamber on an inverted microscope. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm [peak emissions of Ca 2+ bound and free forms]) using a photon counting system. |
Molecular Weight | 730.63 |
Formula | C33H34N2O17 |
Cas No. | 130926-94-2 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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