Mgc(3Me)FDA, a derivative of fluorescein diacetate (FDA) that incorporates a cell-permeable myrGC 3Me motif, effectively penetrates cells. Once inside, it transforms into the fluorescently active mgc(3Me)FL. This compound specifically accumulates in the Golgi apparatus, serving as a visualized Golgi probe [1].

mgc(3Me)FDA, when applied at 10 μM for 10 minutes to HeLa cells, localizes with the Golgi apparatus around the cell nucleus and exhibits green fluorescence [1]. At a concentration of 2.5 μM for 30-270 minutes, mgc(3Me)FDA allows visualization of dynamic morphological changes in the Golgi apparatus during Brefeldin A-induced disassembly in live HeLa cells [1]. Immunofluorescence studies conducted on human epithelial HeLa cells showed fluorescence mainly in the perinuclear Golgi region, co-localizing with the Golgi-tagged fluorescent protein mCherry-Giantin at a concentration of 10 μM for an incubation time of 10 minutes.