ML338 is a selective small molecule inhibitor probe specifically targeting non-replicating Mycobacterium tuberculosis bacilli. It exhibits potent activity against non-replicating M. tuberculosis, with IC90 and IC99 values of 1 μM and 4 μM, respectively, as determined by CFU. This compound, ML338, is an invaluable tool for identifying essential functions and vulnerabilities of M. tuberculosis bacilli under nutrient deprivation conditions. Additionally, ML338 is highly useful for studying M. tuberculosis chemotherapy.

Non-replicatingMycobacterium tuberculosis bacilli:1 μM (IC90), Non-replicatingMycobacterium tuberculosis bacilli:4 μM (IC99)

ML338, within a concentration range of 0-50 μM, demonstrates potency and selectivity towards non-replicating Mycobacterium tuberculosis, achieving an IC90 of 1 μM and exhibiting remarkable efficacy with an IC99 of 4 μM, which is superior in comparison to existing tuberculosis (TB) therapies[1]. Moreover, at concentrations up to 50 μM over 48 hours, ML338 shows no toxicity towards J774 macrophage cells, indicating a high safety profile at the maximal tested concentration[1]. Additionally, ML338 exhibits effective antibacterial activity against both non-replicating and replicating Mycobacterium tuberculosis, with IC90 and IC99 values of 1 μM and 4 μM for the former, and 62 μM for both values in the latter case, as determined by colony-forming unit (CFU) assays[1].