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Monochlorobimane

Catalog No. T19028Cas No. 76421-73-3
Alias mBBr, Chlorobimane

Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.

Monochlorobimane

Monochlorobimane

Purity: 99%
Catalog No. T19028Alias mBBr, ChlorobimaneCas No. 76421-73-3
Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.
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Purity:99%
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Product Introduction

Bioactivity
Description
Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.
In vitro
A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. The Monochlorobimane approach gives comparable results to the HPLC technique. Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content.[1]
AliasmBBr, Chlorobimane
Chemical Properties
Molecular Weight226.66
FormulaC10H11ClN2O2
Cas No.76421-73-3
Storage & Solubility Information
Storagestore at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 45.0 mg/mL (198.5 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM4.4119 mL22.0595 mL44.1189 mL220.5947 mL
5 mM0.8824 mL4.4119 mL8.8238 mL44.1189 mL
10 mM0.4412 mL2.2059 mL4.4119 mL22.0595 mL
20 mM0.2206 mL1.1030 mL2.2059 mL11.0297 mL
50 mM0.0882 mL0.4412 mL0.8824 mL4.4119 mL
100 mM0.0441 mL0.2206 mL0.4412 mL2.2059 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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