Nattokinase is a powerful fibrinolytic enzyme that effectively hydrolyzes fibrin and plasmin substrate, leading to the breakdown of blood clots. Its efficacy renders it valuable for cardiovascular disease studies.

Nattokinase dissolves 94% blood clot at ten minutes and degrades the fibrin clot after two hours of incubation[2].

Nattokinase, administered via oral gavage at dosages of 150-250 mg/kg twice daily for 2 days, demonstrated significant fibrinolytic activity by dissolving blood clots in vivo. In the study using Sprague-Dawley rats weighing 180-220 g and injected with κ-Carrageenan, the treatment not only increased the plasma concentration of fibrin/fibrinogen degradation products (FDPs) and D-Dimer in a dose-dependent fashion but also reduced the area of the vessel cross-section affected by thrombosis.