Tetramethylrhodamine-5-maleimide is a versatile dye utilized in biological research for observing cell structures, tracking and detecting biomolecules, differentiating cell types, evaluating cell functions, studying tissue pathology, and monitoring microorganisms. Its applications extend from fundamental scientific studies to clinical diagnostics. Furthermore, dyes are extensively employed in traditional areas like textile dyeing and innovative fields such as functional textile processing, food pigments, and dye-sensitized solar cells.