Topoisomerase I inhibitor 4 (compound 7a) is a potent inhibitor of topoisomerase I activity, effectively inhibiting the proliferation of various cancer cell lines, including HepG2, A549, MCF-7, and HeLa, with IC50 values of 1.20 μM, 2.09 μM, 1.56 μM, and 1.92 μM, respectively. Thus, Topoisomerase I inhibitor 4 holds promise for cancer research applications [1].

Topoisomerase I inhibitor 4, at concentrations ranging from 1-80 μM, demonstrates Top1 inhibitory activity, while at a dosage of 0-40 μM over 48 hours, it restricts the proliferation of various cancer cell lines, including HepG2, A549, MCF-7, and HeLa. The Cell Proliferation Assay reveals that this compound exhibits significant antiproliferative effects on these cells, with IC 50 values of 1.20 μM for HepG2, 2.09 μM for A549, 1.56 μM for MCF-7, and 1.92 μM for HeLa, respectively.