4-OT Protein, Pseudomonas putida, Recombinant (His)

Catalog No. TMPH-03185

Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate. 4-OT Protein, Pseudomonas putida, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 10.9 kDa and the accession number is Q93JW0.

4-OT Protein, Pseudomonas putida, Recombinant (His)

Catalog No. TMPH-03185

Pack Size	Price	Availability
20 μg	$360	20 days
100 μg	$678	20 days
1 mg	$2,300	20 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate. 4-OT Protein, Pseudomonas putida, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 10.9 kDa and the accession number is Q93JW0.
Species	Pseudomonas putida
Expression System	E. coli
Tag	N-6xHis
Accession Number	Q93JW0
Synonyms	tdnL,4-oxalocrotonate tautomerase,2-hydroxymuconate tautomerase
Amino Acid	PFAQIYMIEGRTEAQKKAVIEKVSQALVEATGAPMANVRVWIQEVPKENWGIAGVSAKELGR
Construction	2-63 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Molecular Weight	10.9 kDa (predicted)
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.

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