APRIL/TNFSF13 Protein, Mouse, Recombinant (His & Flag)

Catalog No. TMPK-01163

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL/TNFSF13 Protein, Mouse, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 18.53 kDa and the accession number is Q9D777.

APRIL/TNFSF13 Protein, Mouse, Recombinant (His & Flag)

Catalog No. TMPK-01163

Pack Size	Price	Availability
100 μg	$487	7-10 days
500 μg	$1,950	7-10 days
1 mg	$3,250	7-10 days

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Product Information

Biological Activity	Immobilized Mouse APRIL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Description	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL/TNFSF13 Protein, Mouse, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 18.53 kDa and the accession number is Q9D777.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-His-Flag
Accession Number	Q9D777
Synonyms	ZTNF2,TRDL-1,TRDL1,TNFSF13,TALL-2,TALL2,CD256,APRILFLJ57090,APRIL
Construction	Ala96-Leu241
Protein Purity	> 90% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Molecular Weight	18.53 kDa (predicted). Due to glycosylation, the protein migrates to 24-30 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.