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Beta-Catenin Protein, Human, Recombinant (His & GST)

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Beta-Catenin Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-02205

Beta-Catenin Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 113 kDa and the accession number is P35222-1.

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Pack Size	Price	Availability
100 μg	$386	In Stock
200 μg	$660	7-10 days
500 μg	$1,340	7-10 days

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Biological Description

Biological Information	Testing in progress
Description	Beta-Catenin Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 113 kDa and the accession number is P35222-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P35222-1
Synonyms	catenin beta 1,armadillo,CTNNB,MRD19,catenin β1,β-Catenin
Construction	The Human CTNNB1 (P35222-1) (Met 1-Leu 781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
Molecular Weight	113 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 150 mM NaCl, 25% glycerol, pH 8.0, 0.1 mM EDTA, 1 mM TCEP, 0.4 mM PMSF, 0.5 mM GSH.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.