1. Loaded Anti-Human CD20 mAb-Fc on AHC Biosensor, can bind Human CD20-Flag with an affinity constant of 3.47 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Human CD20-Flag at 2μg/ml (100 μl/well) can bind Anti-Human CD20 mAb . The ED50 of Anti-Human CD20 mAb is 11.09 ng/ml. (Regularly tested)

CD20 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 33-37 kDa and the accession number is P11836.

Human

HEK293 Cells

MS4A1,Membrane-spanning 4-domains subfamily A member 1,Leukocyte surface antigen Leu-16,CD20,Bp35,B-lymphocyte surface antigen B1,B-lymphocyte antigen CD20

Met1-Pro297

Met1-Pro297

Greater than 70% as determined by reducing SDS-PAGE. (QC verified)

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.