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CES5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.6 kDa and the accession number is Q8BK48.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $600 | 7-10 days |
Biological Activity | Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >50,000 pmoles/min/μg
. |
Description | CES5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.6 kDa and the accession number is Q8BK48. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q8BK48 |
Synonyms | Ces5,carboxylesterase 2E,9030624L02Rik |
Construction | A DNA sequence encoding the extracellular domain of mouse CES5 (NP_766347.1) (Met 1-His 556) was expressed, with a C-terminal polyhistidine tag. Predicted N terminal: Gln 27 |
Protein Purity | > 88 % as determined by SDS-PAGE |
Molecular Weight | 60.6 kDa (predicted); 52 kDa (reducing condition, due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 150 mM NaCl, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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