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CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $398 | 7-10 days |
Biological Activity | Kinase activity untested |
Description | CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). |
Species | Human |
Expression System | E. coli |
Tag | N-His |
Accession Number | AAH07462.1 |
Synonyms | M-CK,creatine kinase, muscle,CKMM |
Construction | A DNA sequence encoding the human CKM (AAH07462.1) (Met1-Lys381) was expressed with a polyhistide tag at the N-terminus. Predicted N terminal: His |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | 44.9 kDa (predicted); 45 kDa (reducing conditions) |
Endotoxin | Please contact us for more information. |
Formulation | Supplied as sterile 100 mM HEPES, pH 7.0. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | Shipping with blue ice. |
Research Background | CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). |
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