CXCL4 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00013

Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes. CXCL4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 10.68 kDa and the accession number is P02776.

CXCL4 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00013

Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Immobilized Human CCL5, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1μg/ml determined by ELISA.
Description	Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes. CXCL4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 10.68 kDa and the accession number is P02776.
Species	Human
Expression System	E. coli
Tag	N-His-Avi
Accession Number	P02776
Synonyms	SCYB4,PF-4,Oncostatin-A,MGC138298,Iroplact,CXCL4
Construction	Glu32-Ser101
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	10.68 kDa (predicted). The protein migrates to 13 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 4 mM HCl (pH 3.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 4mM HCl (pH 3.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.