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DNA ligase Protein, Desulfotalea psychrophila, Recombinant (His) is expressed in E. coli.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $237 | 20 days | |
100 μg | $446 | 20 days | |
1 mg | $1,920 | 20 days |
Biological Activity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | DNA ligase Protein, Desulfotalea psychrophila, Recombinant (His) is expressed in E. coli. |
Species | Desulfotalea psychrophila |
Expression System | E. coli |
Tag | N-10xHis |
Accession Number | Q6API9 |
Synonyms | Polydeoxyribonucleotide synthase [NAD(+)],ligA,DNA ligase |
Amino Acid | RPQTSRLGRVNPVAEVEPTRLSGALIQRVTAHHYAMVRDRGIGPGARIRMSRSGEVIPKIEEVVKPVAAAQLDIPAHCPSCGSDLAWDGDYLFCLNNMSCPAQITNSMEHFFKILGNVDGFGPSSVQRLYEGGLVDLPALYAMESADFEQVGFGPKQATNMVEQLNRSREELIEDWRFLAAFGVHRLGMGNCEKLLRYVRLEDVFNLSEEQIVAEIKGFQEKTAHSICAGLAGIKDLFFQLFALGFKLEPTPLLAEGGQGEKSPISGKTIVFTGAMLAASRGDMEKQAKALGATVGKSITGKTDMLVTGQRVGATKMAKAETLGIAILSEEAYRQLVSLD |
Construction | 340-679 aa |
Protein Purity | > 85% as determined by SDS-PAGE. |
Molecular Weight | 40.4 kDa (predicted) |
Formulation | Tris-based buffer, 50% glycerol |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
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