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EGFL6 Protein, Human, Recombinant (His)

Catalog No. TMPY-03780

EGFL6 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 60.5 kDa and the accession number is Q8IUX8-1.

EGFL6 Protein, Human, Recombinant (His)

EGFL6 Protein, Human, Recombinant (His)

Catalog No. TMPY-03780
EGFL6 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 60.5 kDa and the accession number is Q8IUX8-1.
Pack SizePriceAvailabilityQuantity
50 μg$3987-10 days
500 μg$2,5107-10 days
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Product Information

Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to EGFL6-His coated plates (5μg/mL and 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.
Description
EGFL6 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 60.5 kDa and the accession number is Q8IUX8-1.
Species
Human
Expression System
Baculovirus Insect Cells
TagC-His
Accession NumberQ8IUX8-1
Synonyms
W80,MAEG,EGF-like-domain, multiple 6
Construction
A DNA sequence encoding the human EGFL6 (NP_056322.2)(Met1-Asp553) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Asn 22
Protein Purity
> 60 % as determined by SDS-PAGE
Molecular Weight60.5 kDa (predicted); 62 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 500 mM NaCl, 10% Glycerol, pH7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.

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