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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $386 | In Stock | |
200 μg | $697 | 7-10 days | |
500 μg | $1,520 | 7-10 days |
Biological Information | 1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His , the EC50 of Human EGFR His is 100-500 ng/mL.
2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 nM as determined in an SPR assay (Routinely tested).
3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag with an affinity constant of 5.81nM as determined in BLI assay (Routinely tested). ![]() ![]() |
Description | EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | P00533-1 |
Synonyms | mENA,NISBD2,ERBB1,HER1,PIG61,ERBB,epidermal growth factor receptor |
Construction | extracellular domain (Met 1-Ser 645) of Human EGFR (NP_005219) |
Protein Purity | > 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC ![]() ![]() |
Molecular Weight | 69.8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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