Fc gamma RIIIA/CD16a (F176) Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00792

Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. Fc gamma RIIIA/CD16a (F176) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 24.7 kDa and the accession number is P08637-1.

Fc gamma RIIIA/CD16a (F176) Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00792

Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Product Information

Biological Activity	1. Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 35.7ng/ml determined by ELISA (QC Test). 2. Biotinylated Human Fc gamma RIIIA (F176), His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14μM as determined in SPR assay.
Description	Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. Fc gamma RIIIA/CD16a (F176) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 24.7 kDa and the accession number is P08637-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P08637-1
Synonyms	IMD20,IGFR3,FcRIIIa,FcRIII,FcR-10,FcgRIIIA,FCGRIII,FCGR3A,FCGR3,FCG3,Fc γ RIIIA/CD16a (F176),Fc γ RIIIA,Fc gamma RIIIA,CD16A,CD16
Construction	Gly17-Gln208(F176)
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	24.7 kDa (predicted). Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.