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Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $462 | 7-10 days | |
100 μg | $1,070 | 7-10 days |
Biological Information | 1.Loaded Biotinylated Human FcγRIIIA / CD16a (F176) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.26 μM as determined in a BLI assay.2.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Biotinylated Human FcγRIIIA / CD16a (F176) recombinant protein (His & Avi Tag) with an affinity constant of 0.23 μM as determined in a BLI assay.3. The purity of Human CD16a / FCGR3A Protein (176 Phe, His & AVI Tag), Biotinylated is more than 95% and the molecular weight of this protein is around 31-47 kDa verified by SEC-MALS. |
Description | CD16a Protein, Human, Recombinant (176F, His & Avi), biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.1 kDa and the accession number is P08637-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-Avi-His |
Accession Number | P08637-1 |
Synonyms | CD16A,FCG3,FCGRIII,FCR-10,FCRIIIA,Fc gamma RIIIa,CD16,FCRIII,Fc γ RIIIa,Fc fragment of IgG, low affinity IIIa, receptor (CD16a),IGFR3,IMD20,FCGR3 |
Construction | The extracellular domain (Met1-Gln208) of Human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus (AVI-his). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC |
Molecular Weight | 25.1 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
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