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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $386 | In Stock | |
200 μg | $682 | 7-10 days | |
500 μg | $2,240 | 7-10 days |
Biological Information | 1. Immobilized Human FcγRIIIA / CD16a (V176) recombinant protein at 2 μg/mL can bind Human IgG1 , the EC50 is 150-450 ng/mL._x000D_
2.Loaded Human FcγRIIIA / CD16a (V176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.83 μM as determined in a BLI assay ._x000D_
3.Captured Human FcγRIIIA / CD16a (V176) recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 μM as determined in an SPR assay._x000D_
4.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (V176) recombinant protein with an affinity constant of 0.78 µM as determined in an SPR assay. |
Description | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells._x000D_
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | P08637-1 |
Synonyms | IMD20,IGFR3,FCRIIIA,FCRIII,FCR-10,FCGRIII,FCGR3,FCG3,Fc γ RIIIa,Fc gamma RIIIa,Fc fragment of IgG, low affinity IIIa, receptor (CD16a),CD16A,CD16 |
Construction | A DNA sequence encoding the extracellular domain (Met1-Gln208) of human CD16a (P08637-1, with natural variant Phe 176 Val) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gly 17 |
Protein Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC |
Molecular Weight | 23.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells._x000D_
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
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