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CD16b Protein, Human, Recombinant (NA2, His)

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CD16b Protein, Human, Recombinant (NA2, His)

Catalog No. TMPY-00701

CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.

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Pack Size	Price	Availability
100 μg	$386	In Stock
200 μg	$682	7-10 days
500 μg	$1,440	7-10 days

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Biological Description

Biological Information	1.Loaded Human FcγRIIIB / CD16b (NA2) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 8.3 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested). 2.Captured Human FcγRIIIB / CD16b (NA2) recombinant protein on Anti-His Chip can bind IgG1 with an affinity constant of 2.6 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 3.Captured IgG1 on proteinA Chip can bind Human FcγRIIIB/CD16b (NA2) recombinant protein with an affinity constant of 0.22 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.The purity of Human CD16b/FCGR3B Protein (His Tag, NA2 allotype) is more than 95% and the molecular weight of this protein is around 33-43 kDa verified by SEC-MALS.
Description	CD16b Protein, Human, Recombinant (NA2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.2 kDa and the accession number is O75015.2.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	O75015.2
Synonyms	Fc gamma RIIIb,FCR-10,FCRIIIb,Fc γ RIIIb,CD16b,CD16,FCG3,FCRIII,Fc fragment of IgG receptor IIIb,FCGR3
Construction	The Human CD16b (O75015.2) (Met 1-Ser 200) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
Molecular Weight	22.2 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.