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CD16a Protein, Human, Recombinant (176 Phe, His & Avi)

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CD16a Protein, Human, Recombinant (176 Phe, His & Avi)

Catalog No. TMPY-01965

CD16a Protein, Human, Recombinant (176 Phe, His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.1 kDa and the accession number is P08637-1.

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Pack Size	Price	Availability	Quantity
100 μg	$600	7-10 days
1 mg	$3,910	7-10 days

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Biological Description

Biological Information	Measured by its binding ability in a functional ELISA. Immobilized Human CD16a His & AVI at 2 μg/ml (100 μl/well) can bind Human IgG1 , the EC50 of Human IgG1 is 0.4-1.2 μg/mL.
Description	CD16a Protein, Human, Recombinant (176 Phe, His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.1 kDa and the accession number is P08637-1.
Species	Human
Expression System	HEK293 Cells
Tag	His, Avi
Accession Number	P08637-1
Synonyms	IMD20,IGFR3,FCGR3,Fc fragment of IgG, low affinity IIIa, receptor (CD16a),Fc γ RIIIa,CD16,FCR-10,FCG3,CD16A,FCRIIIA,FCGRIII,Fc gamma RIIIa,FCRIII
Construction	The extracellular domain (Met 1-Gln 208) of Human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	25.1 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.2. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.