1. Measured by its ability to bind human IgG1 in functional ELISA.
_x000D_ 2. Loaded Cynomolgus FcRn recombinant protein on His1K Biosensor, can bind IgG1 Fc with an affinity constant of 0.032 μM as determined in a BLI assay.
_x000D_ 3. Captured Cynomolgus FcRn recombinant protein  on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.09 µM as determined in an SPR assay.
_x000D_ 4. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.18 µM as determined in an SPR assay (Biacore T200).
_x000D_ 5. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein is more than 95% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is Q8SPV9&Q8SPW0.

Cynomolgus

HEK293 Cells

Fc fragment of IgG, receptor, transporter, alpha,Fc fragment of IgG, receptor, transporter, α

The extracellular domain (Met 1-Ser 297) of cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.