1. Measured by its ability to bind human IgG1 in a functional ELISA.
2. Loaded Human FcRn recombinant protein on His1K Biosensor, can bind IgG1 with an affinity constant of 0.11 µM as determined in a BLI assay.
3. Loaded Human FcRn recombinant protein on His1k Biosensor, can bind IgG4 Fc with an affinity constant of 0.08 µM as determined in a BLI assay.
4. Captured Human FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 µM as determined in an SPR assay (Biacore T200).
5. Captured  Human FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 µM as determined in an SPR assay (Biacore T200).
6. The purity of Human FCGRT & B2M Heterodimer Protein is more than 90% and the molecular weight of this protein is around 45-53 kDa verified by SEC-MALS.
7. Loaded Recombinant Human FCGRT & B2M Heterodimer Protein, His Tag on His1K Biosensor, can bind Anti-Erbb2 Antibody, human IgG1 with an affinity constant of 11.1nM as determined in BLI assay (QC tested).

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is P55899-1&P61769.

Human

HEK293 Cells

Fc fragment of IgG, receptor, transporter, alpha,alpha-chain,α-chain,Fc fragment of IgG, receptor, transporter, α,FCRN

The extracellular domain (Met1-Ser297) of Human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The Human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.