1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA. 2.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG1 Fc (IgG1) with an affinity constant of 0.073 μM as determined in a BLI assay. 3.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG4 Fc with an affinity constant of 0.070 μM as determined in a BLI assay. 4. The purity of Human FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 95% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is NP_004098.1&NP_004039.1.

Human

HEK293 Cells

α-chain,FCRN,Fc fragment of IgG, receptor, transporter, α,Fc fragment of IgG, receptor, transporter, alpha,alpha-chain

A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.