1.Measured by its ability to bind recombinant rhFc(CHO) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 0.4 nM as determined in a BLI assay.

FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 46 kDa and the accession number is Q61559.

Mouse

HEK293 Cells

FcRn,Fc fragment of IgG, receptor, transporter, α,Fc fragment of IgG, receptor, transporter, alpha

A DNA sequence encoding the mouse FCGRT (NP_034319.2) (Met 1-Ser 297) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ser 22 & Ile 21

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.