FGF-21 Protein, Human, Recombinant (mFc & Avi)

Catalog No. TMPK-00097

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia. FGF-21 Protein, Human, Recombinant (mFc & Avi) is expressed in HEK293 mammalian cells with N-mFc-Avi tag. The predicted molecular weight is 46.9 kDa and the accession number is Q9NSA1-1.

FGF-21 Protein, Human, Recombinant (mFc & Avi)

Catalog No. TMPK-00097

Pack Size	Price	Availability
100 μg	$581	7-10 days
500 μg	$2,330	7-10 days
1 mg	$3,880	7-10 days

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Product Information

Biological Activity	1. Immobilized Human FGF21 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29μg/ml determined by ELISA (QC Test). 2. Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay.
Description	Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia. FGF-21 Protein, Human, Recombinant (mFc & Avi) is expressed in HEK293 mammalian cells with N-mFc-Avi tag. The predicted molecular weight is 46.9 kDa and the accession number is Q9NSA1-1.
Species	Human
Expression System	HEK293 Cells
Tag	N-mFc-Avi
Accession Number	Q9NSA1-1
Synonyms	UNQ3115,PRO10196,FGF-21,FGF21
Construction	His29-Ser209
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	46.9 kDa (predicted). Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.