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Fragilysin Protein, Bacteroides fragilis, Recombinant (His)

Catalog No. TMPH-00190

Diarrheal toxin that hydrolyzes gelatin, azocoll, actin, tropomyosin, and fibrinogen. Fragilysin Protein, Bacteroides fragilis, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 46.7 kDa and the accession number is P54355.

Fragilysin Protein, Bacteroides fragilis, Recombinant (His)

Fragilysin Protein, Bacteroides fragilis, Recombinant (His)

Catalog No. TMPH-00190
Diarrheal toxin that hydrolyzes gelatin, azocoll, actin, tropomyosin, and fibrinogen. Fragilysin Protein, Bacteroides fragilis, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 46.7 kDa and the accession number is P54355.
Pack SizePriceAvailabilityQuantity
20 μg$36020 days
100 μg$67820 days
1 mg$2,30020 days
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Product Information

Biological Activity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Diarrheal toxin that hydrolyzes gelatin, azocoll, actin, tropomyosin, and fibrinogen. Fragilysin Protein, Bacteroides fragilis, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 46.7 kDa and the accession number is P54355.
Species
Bacteroides fragilis
Expression System
E. coli
TagN-6xHis
Accession NumberP54355
Synonyms
Fragilysin,Enterotoxin,btfP
Amino Acid
ACSNEADSLTTSIDAPVTASIDLQSVSYTDLATQLNDVSDFGKMIILKDNGFNRQVHVSMDKRTKIQLDNENVRLFNGRDKDSTSFILGDEFAVLRFYRNGESISYIAYKEAQMMNEIAEFYAAPFKKTRAINEKEAFECIYDSRTRSAGKDIVSVKINIDKAKKILNLPECDYINDYIKTPQVPHGITESQTRAVPSEPKTVYVICLRENGSTIYPNEVSAQMQDAANSVYAVHGLKRYVNFHFVLYTTEYSCPSGDAKEGLEGFTASLKSNPKAEGYDDQIYFLIRWGTWDNKILGMSWFNSYNVNTASDFEASGMSTTQLMYPGVMAHELGHILGAEHTDNSKDLMYATFTGYLSHLSEKNMDIIAKNLGWEAADGD
Construction
26-405 aa
Protein Purity
> 90% as determined by SDS-PAGE.
Molecular Weight46.7 kDa (predicted)
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Diarrheal toxin that hydrolyzes gelatin, azocoll, actin, tropomyosin, and fibrinogen.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Please read the User Guide of Recombinant Proteins for more specific information.