1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 10-50 ng/mL in the presence of Recombinant Rat GFR alpha‑1/GDNF R alpha‑1 His Chimera. 2. Immobilized rat His-GDNF (78-211) at 10 μg/ml (100 μl/well) can bind rat GFRA1-Fc , The EC50 of rat GFRA1-Fc is 20-46.6 ng/ml.

GDNF Protein, Rat, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 17.1 kDa and the accession number is Q07731-1.

Rat

Baculovirus Insect Cells

glial cell derived neurotrophic factor

A DNA sequence encoding the mature form of rat GDNF (Q07731-1) (Ser78-Ile211) was expressed,with a polyhistide tag at the N-terminus. Predicted N terminal: His

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.