**HLA-A*02:01 & B2M & MART-1 (ELAGIGILTV) Monomer Protein, Cynomolgus, Recombinant (His)**

Catalog No. TMPK-00650

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. HLA-A*02:01 & B2M & MART-1 (ELAGIGILTV) Monomer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 19.78 kDa and the accession number is G7NVB0.

**HLA-A*02:01 & B2M & MART-1 (ELAGIGILTV) Monomer Protein, Cynomolgus, Recombinant (His)**

Catalog No. TMPK-00650

Pack Size	Price	Availability
100 μg	$487	7-10 days
500 μg	$1,950	7-10 days
1 mg	$3,250	7-10 days

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Product Information

Biological Activity	1. Immobilized Cynomolgus IL-10, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 71.2ng/ml determined by ELISA (QC Test). 2. Cynomolgus IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-10, His Tag with an affinity constant of 0.93 nM as determined in SPR assay.
Description	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. HLA-A*02:01 & B2M & MART-1 (ELAGIGILTV) Monomer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 19.78 kDa and the accession number is G7NVB0.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	N-His
Accession Number	G7NVB0
Synonyms	TGIF,MGC126451,MGC126450,Interleukin-10,IL10A,IL-10,GVHDS,CSIF,B-TCGF
Construction	Ser19-Asn178
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	19.78 kDa (predicted). Due to glycosylation, the protein migrates to 20-22 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.