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IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST)

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IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST)

Catalog No. TMPY-06179

IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus. IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST) is expressed in E. coli expression system with His and GST tag. The predicted molecular weight is 63.1 kDa and the accession number is F8V4V0-1.

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Pack Size	Price	Availability	Quantity
20 KU	$2,170	7-10 days

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Biological Description

Biological Information	One unit digests ≥ 95% of 1µg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.
Description	IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus. IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST) is expressed in E. coli expression system with His and GST tag. The predicted molecular weight is 63.1 kDa and the accession number is F8V4V0-1.
Species	Streptococcus pyogenes
Expression System	E. coli
Tag	His, GST
Accession Number	F8V4V0-1
Construction	The streptococcus pyogenes IdeS (F8V4V0-1) (Asp30-Asn341) was expressed with the N-terminal polyhistidine-tagged a GST tag at the N-terminus (his-GST)
Protein Purity	> 85 % as determined by SDS-PAGE.
Molecular Weight	63.1 kDa (predicted)
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, 50% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus.

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