IFN gamma Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00047

Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579.

IFN gamma Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00047

Pack Size	Price	Availability
100 μg	$184	7-10 days
500 μg	$745	7-10 days
1 mg	$1,240	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Immobilized Human IFN gamma, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 32.7 ng/mL determined by ELISA.
Description	Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P01579
Synonyms	γ,Interferon-γ,Interferon-gamma,interferon,IFNγ,IFN γ/IFNG,IFN γ,IFN gamma,IFI,IFG,gamma
Construction	Gln24-Gly161
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	19.1 kDa (predicted). Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.