1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IL10RA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human IL10 , The EC50 of biotinylated human IL10 is 39.0-92.0 ng/ml. 2. Measured by its ability to inhibit IL-10 dependent proliferation of MC/9 mouse mast cells. Approximately 0.1-0.4 μg/mL of IL-10Rα will inhibit 50% of the biological response induced by 10 ng/mL of recombinant human IL-10.

IL-10R alpha/IL-10RA Protein, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.8 kDa and the accession number is XP_001092736.1.

Rhesus

HEK293 Cells

interleukin 10 receptor, α,interleukin 10 receptor, alpha

A DNA sequence encoding the rhesus IL10RA (XP_001092736.1) (Thr9-Asn245) was expressed, fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Phe 29

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

IL10RA (Interleukin 10 Receptor Subunit Alpha, also known as CD210) is a Protein Coding gene. The protein encoded by this gene is a receptor for interleukin 10. Two transcript variants, one protein-coding, and the other, not protein-coding have been found for this gene. CD210 belongs to the type II cytokine receptor family. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. Activation of CD210 leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. CD210 is broadly expressed in the spleen, bone marrow, and other tissues. Diseases associated with IL10RA include Inflammatory Bowel Disease 28, Autosomal Recessive, and Inflammatory Bowel Disease 28.