1. Measured by its binding ability in a functional ELISA. 2. Immobilized rat IL17F-His at 10 μg/mL (100 μL/well) can bind Rat IL17RA-Fc3 . The EC50 of Rat IL17RA-Fc3 is 0.28-0.66 μg/mL.

IL-17F Protein, Rat, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 16.4 kDa and the accession number is Q5BJ95.

Rat

Baculovirus Insect Cells

interleukin 17F

A DNA sequence encoding the rat Il17f (NP_001015011.1) (Met1-Ala153) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 19

> 90 % as determined by SDS-PAGE.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is its involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also functions in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and the exacerbation of antigen-induced pulmonary allergic inflammation.