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IL-17F Protein, Mouse, Recombinant (His & Avi)

Catalog No. TMPK-00041
IL-17F Protein, Mouse, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 17.8 kDa and the accession number is Q7TNI7-1.
Pack SizePriceAvailabilityQuantity
100 μg$487In Stock
500 μg$1,9507-10 days
1 mg$3,2507-10 days
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Product Information

Biological Activity
Immobilized Mouse IL-17F, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 1.25μg/ml determined by ELISA.
Description
IL-17F Protein, Mouse, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 17.8 kDa and the accession number is Q7TNI7-1.
Species
Mouse
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberQ7TNI7-1
Synonyms
ML1,IL-24,IL24,IL-17F,IL17F,Cytokine ML-1,CANDF6
Construction
Arg29-Ala161
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight17.8 kDa (predicted). Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.

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