1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/ml (100ul/well) can bind recombinant human IL1R2-Fc with a linear range of 6.25-400 ng/ml. 2. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) bound IL-1 beta Protein, Human, Recombinant was 6.0 nM. 3. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The EC50 for this effect is typically 0.1-0.8 μg/mL.

IL-1R2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.5 kDa and the accession number is P27930-1.

Human

HEK293 Cells

interleukin 1 receptor, type II,IL-1RT-2,IL-1RT2,IL1RB,IL1R2c,IL-1R-2,CDw121b,CD121b

A DNA sequence encoding the extracellular domain (Met 1-Glu 343) of human IL1 receptor type I I (IL1RII) (NP_004624.1) was expressed with C-terminal fused Fc region of human IgG1. Predicted N terminal: Phe 14

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.