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IL-12 Protein, Human, Recombinant (His), Biotinylated

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IL-12 Protein, Human, Recombinant (His), Biotinylated

Catalog No. TMPY-05458

IL-12 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459.2&NP_002178.2.

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Pack Size	Price	Availability	Quantity
20 μg	$383	7-10 days
100 μg	$913	7-10 days

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Biological Description

Biological Information	Testing in progress
Description	IL-12 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459.2&NP_002178.2.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P29459.2&NP_002178.2
Synonyms	NKSF,IL-12A,interleukin 12A,IMD29,NFSK,NKSF2,CLMF2,NKSF1,IMD28,IL-12B,IL12 p70,IL12,P35,CLMF
Construction	The p35 subunit of Human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; The p40 subunit of Human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. The purified protein was biotinylated in vitro.
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	60.13 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.